|
This study was carried out to detect spontaneous mouse hepatitis virus (MHV) infection of mice in a breeding colon5 in Korea by pathological and immunohistochemical methods. ICR mice, 6 weeks old (female 1, male 1) were found to be sudden death during the experiment, Grossly, the liver appeared dark red color, scattered with the white pin-point spots, Multi-focal hepatocellular necrosis was seen in the liver without preference for any particular none of the liver lobule. A focal hepatocellular necrosis was infiltrated with inflammatory cells, For identifying MHV antigen, an immunahistochemical staining method using avidin-biotin-paroxidase complex (ABC) was applied in formaln-fixed, paraffin-embedded tissues. Positive reactions against MHV antigen were shown in many hepatocytes or the lesions of focal hepatocellular necrosis. In conclusion, mice that were spontaneously infected with a strain of MHV in domestic breeding system were detected by pathological and immunohistochemical(ABC) methods. This technique can be used for the definitive diagnosis of an active mouse hepatitis virus infection using commercially available reagents and formalin-fixed, paraffin-embedded tissues.
|